| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 441 | Thermobifida fusca | TCTACGCACGCAAAGGCA | GCACGTTGTTCCCGTTGAC | 59.97 | 60.01 | 222 | 105 |
100.00%
|
100.00%
|
| Primer 442 | Thermobifida fusca | ACTTTGCACCCCAGCACA | CCGGACAGTTCAGCGATCT | 59.72 | 59.49 | 184 | 105 |
100.00%
|
100.00%
|
| Primer 443 | Thermobifida fusca | ACAGCCACCGAAGAGGACT | GGCGTATTCCAGTCGAGCA | 60.53 | 59.86 | 296 | 105 |
100.00%
|
100.00%
|
| Primer 444 | Thermobifida fusca | AAGTGGACGACTGCGAGTG | GCGGATTGTGCTCTTTGCC | 60.01 | 60.15 | 174 | 105 |
100.00%
|
100.00%
|
| Primer 445 | Thermobifida fusca | TGACCAACTGCGCACACT | TCGGCTCGTCTTGCAGAT | 59.81 | 58.71 | 216 | 105 |
100.00%
|
100.00%
|
| Primer 446 | Thermobifida fusca | TGACCAACTGCGCACACT | TCAGTGCCTGCTTGTCCTC | 59.81 | 59.63 | 183 | 105 |
100.00%
|
100.00%
|
| Primer 447 | Thermobifida fusca | ATGGCGACCATGGTGTTCC | CGTTGCTCTGGATCCCCAT | 60.38 | 59.47 | 286 | 105 |
100.00%
|
100.00%
|
| Primer 448 | Thermobifida fusca | TGAACCACATCGACCACCC | ACCGCTTCTTGTCGAGCA | 59.63 | 59.27 | 204 | 105 |
100.00%
|
100.00%
|
| Primer 449 | Thermobifida fusca | TCGTTTGTGATGGCGTGC | ACATCGCTAGCGTGTGCA | 59.06 | 59.74 | 220 | 105 |
100.00%
|
100.00%
|
| Primer 450 | Thermobifida fusca | GTCGCGTCGTTTGTGATGG | ACATCGCTAGCGTGTGCA | 59.87 | 59.74 | 226 | 105 |
100.00%
|
100.00%
|